Comparisons of condylar movements with the functional occlusal clutch and tray clutch recording methods in CADIAX system / 国际口腔科学杂志·英文版

<p><b>AIM</b>The purpose of this study is to compare the effects of the two clutches on recording the condylar movement.</p><p><b>METHODOLOGY</b>Ten subjects (6 women, 4 men; mean age 25.4 years) participated in the study. The mandibular movement, sagittal condylar inclination angle, and transversal condylar inclination angle of each subject were recorded with the CADIAX using the two clutches, respectively. The characteristics of the tracings of the protrusion, opening, and mediotrusion were analyzed with the t-test statistics at a = 0.05 level. The Kappa values were calculated for an assessment of the congruence of the tracings.</p><p><b>RESULTS</b>The results showed that the contour, direction, and dimension of the tracings in the two clutches were approximately same, but the tracings determined by the functional occlusal clutch were more regular and congruent. In the group segment recorded with the tray clutch, opening/closing paths of one subject showed crossed and time curves of three subjects appeared peak-like changes of velocity, but none were statistically different (P>0.05).</p><p><b>CONCLUSION</b>The research suggests that the functional occlusal clutch should be preferred in the evaluation of the mandibular function, as the tracings with the tray clutch are more likely to produce false positive results.</p>

Expression of E-cadherin and beta-catenin in oral squamous cell carcinomas of tongue: correlation with the clinicopathologic features and patient prognosis / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To examine the E-cadherin and beta-catenin expression in oral squamous cell carcinoma of tongue (OSCCT) and investigate the relationship of these markers with clinicopathologic features and patient prognosis.</p><p><b>METHODS</b>Quantitative immunohistochemistry analysis was used to examine E-cadherin and beta-catenin expression in lesions of 30 OSCCT patients. The relationship between the expression of E-cadherin and beta-catenin and clinicopathological features was analyzed.</p><p><b>RESULTS</b>The decreased expression of E-cadherin was observed in 19 of 30 (63%) tumours from patients who eventually developed a recurrent tumour and was also associated with recurrence (P=0.007). The expression of E-cadherin was associated with survival (P=0.018) and an independent prognostic factor in univariate analysis. There was no correlation between the expression level of E-cadherin and sex, age, histological differentiation, tumour size, clinical stage, or lymph node metastasis. The high expression of beta-catenin was observed in 18 of 30 (60%) tumours. No correlation between beta-catenin expression and clinicopathological features was observed.</p><p><b>CONCLUSIONS</b>The absence or reduced expression of E-cadherin was closely associated with recurrence and survival in OSCCT patients. The aberrant expression of E-cadherin may provide a useful prognostic marker in OSCCT.</p>

Function of P63 on the development of salivary glands / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the expression of P63 in human salivary gland development and the existing of salivary gland stem cells.</p><p><b>METHODS</b>24 embryonic salivary gland samples in different stage, 10 normal salivary gland samples were collected. HE-stained and immunochemistry stain were used.</p><p><b>RESULTS</b>It could be seen on the HE-stained sections that the epithelial buds proliferated to form the epithelial branches and duct systems, finally the terminal cells differentiated into ductal, myoepithelial and acinous. During the development of salivary gland, the expression of P63 was gradually reduced. In normal adult salivary gland samples, the positive cell interspersed in the basal layer of intercalated duct, secretory duct and excretory duct.</p><p><b>CONCLUSION</b>P63 plays an important role in human salivary gland development. The result of our experiment shows the distributive characteristic of salivary gland stem cells, which exist abroad in bud stage, but decrease and only exist in the basal layer of ducts in normal adult salivary gland.</p>

Evaluation of a model of temporomandibular disorders established by transzygomatic arch traction of the mandibular ramus in rabbits / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate a model of temporomandibular disorders established by transzygomatic arch traction of the mandibular ramus in rabbits.</p><p><b>METHODS</b>Fifteen adult New Zealand rabbits were subjected to traction in the postero-superior direction unilaterally using elastic force and six rabbits used as the control. Histopathologic change of the disc, joint space and cartilage was observed through Hematoxylin and Eosin staining.</p><p><b>RESULTS</b>Anterior disc displacement or disc deformity in four experimental rabbits was observed on the traction side 2 weeks after operation. At 4 weeks, fibrous adhesions in joint compartment were found in five experimental rabbits. The condyles or articular eminences of some experimental rabbits showed irregularities on the cartilage surface. In the 6 th week, bad disc deformity in four rabbits and severe fibrous adhesions in five rabbits was observed on the traction side, and subchondralbone and calcified cartilage became irregular. In control group, All articular structures were normal.</p><p><b>CONCLUSIONS</b>A animal model of temporomandibular disorders can be established by transzygomatic arch traction of the mandible.</p>

Regulation of PTHrP in proliferation and differentiation of chondrocytes of condyle in fetal mouse / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the regulatory mechanism of parathyroid hormone-related protein (PTHrP) in proliferation and differentiation of chondrocytes of condyle in fetal mouse.</p><p><b>METHODS</b>Chondrocytes of condyle in fetal mouse were separated and cultured in vitro, the influence of PTHrP on proliferation and differentiation was observed.</p><p><b>RESULTS</b>After two weeks' culture in 0.01 nmol/L, 0.1 nmol/L, 1 nmol/L, 10 nmol/L human PTHrP, there was significant difference in the number of cartilage nodule formed between experiment group and control group (P<0.05), and there was no significant difference in 0.01 nmol/L group (P>0.05). Alkaline phosphatase (ALP) activity was significantly intensified in experiment group and control group (P<0.05). Meanwhile, it was found that this function of promotion was lessened after anti-PTHR antibody used.</p><p><b>CONCLUSION</b>It can be seen that PTHrP, via its receptor, can promote proliferation and differentiation of chondrocytes of condyle, which resemble its modulation mechanism in epiphyseal growth plate cartilage intramembrane in mandibule.</p>

The distribution of collagen I, II, X and alkaline phosphatase in the development of condylar cartilage of fetal mouse mandible / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study was to investigate the distribution of collagen I, II , X, alkaline phosphatase (ALP) and their roles during initiation of condylar cartilage of the fetal mouse.</p><p><b>METHODS</b>Coronary sections of mandible of mouse embryo aged from 14th to 18th day were studied under light microscope after stained by immunohistochemical method with antibody of types I, II, X collagen and ALP.</p><p><b>RESULTS</b>On the 14th day of mouse embryo, it was found that mesenchymal cells condensation continuous with the periosteum. Type I collagen and ALP were positive behind the terminal of the ossifying mandibular periosteum where future condylar will form. On the 15th day, positive staining for types I, II collagen was found in mesenchymal cells around hypertrophic cells and type X collagen was detected in hypertrophic cells. ALP was positive in both mesenchymal cells and hypertrophic cells. On the 16th day, type I collagen was observed from periosteal osteogenic cells and mesenchymal cells of the fibrous cell layer to the upper hypertrophic cell layer while Type II collagen was restricted from the lower polymorphic cell layer to the bottom of the hypertropic cell layer. Type X collagen was positive in the hypertrophic cell layer. ALP was positive in periosteal osteogenic cells and hypertrophic chondral cells, but not in the polymorphic cell layer.</p><p><b>CONCLUSION</b>Development of condylar cartilage is different from that of limb bone. Types I, II, X collagen are expressed in the condylar chondrocyte on the early stage of endochondral ossification. The histology evidence supports the conjecture that condylar cartilage is derived from differentiated mesenchymal cells of the preperiosteum or periosteum of the mandible where ALP is positively expressed.</p>

Clinicopathological study of Ewing's sarcoma/primitive neuroectodermal tumor in oral and maxillofacial region / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To study the clinicopathological features and diagnostic criteria of Ewing's sarcoma/primitive neuroectodermal tumor (Ewing's sarcoma/PNET) in oral and maxillofacial region.</p><p><b>METHODS</b>There were 15 patients with Ewing's sarcoma/PNET in the last 35 years at our hospital, aged 1-49 years and mean 14.5 years. The most common manifestation was swelling of the affected region. The cases were analyzed and histological and immunohistochemical studies were also conducted to examine CD99 (12E7), Vimentin, NSE, S-100, Syn, CD45 (LCA), desmin.</p><p><b>RESULTS</b>(1) The most common histological pattern of Ewing's sarcoma/PNET was a lobular arrangement of uniform, small, hyperchromatic cells in a fibrous background. Some of these tumors were rich in cytoplasmic glycogen. (2) Of the 7 cases, IHC was positive for CD99 (12E7) and Vimentin and negative for lymphoid (CD45), muscle (desmin) markers. S-100 was positive in 4 cases and NSE negative in 3, Syn positive in 1. NSE, S-100, Syn were all negative in 2 cases.</p><p><b>CONCLUSIONS</b>Ewing's sarcoma/PNET is more common in teenagers and young people. Immunohistochemistry is essential to distinguish Ewing's sarcoma/PNET from other small round cell tumors. Immunohistochemistry is useful in the diagnosis.</p>

The correlation between neck lymphy node metastasis and matrixmetalloproteinase-2 expression at the invasive tumor front of oral squamous cell carcinomas / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To explore the correlation between neck lymph node metastasis and matrix metalloproteinase-2 expression at the invasive tumor front of oral squamous cell carcinomas(OSCC).</p><p><b>METHODS</b>Immunohistochemistry LsAB technique was used to observe the expression of MMP-2 at the invasive tumor front and center of OSCC, and the correlation between the expression of MMP-2 in OSCC and neck lymph node metastasis were respectively analyzed by statistics.</p><p><b>RESULTS</b>The results demonstrated that MMP-2 existed in all 71 cases, which the expression of MMP-2 at the OSCC front was more significant than that of MMP-2 at the OSCC center (P < 0.01), and related to neck lymph node metastasis (P < 0.05).</p><p><b>CONCLUSION</b>The expression of MMP-2 at the OSCC front could be considered as an index of judging the present of neck lymph node metastasis of OSCC.</p>

A retrospective study on 615 cases of minor salivary gland tumors / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To analyse the clinical and pathological characteristics of minor salivary gland tumors.</p><p><b>METHODS</b>A retrospective analysis on 615 cases of intraoral minor salivary gland tumors from 1990-2002 with a confirmed pathologic diagnosis was carried out.</p><p><b>RESULTS</b>In 615 cases of minor salivary gland tumors, 265 cases were benign, 350 cases malignant. Pleomorphic adenoma was the most common entity and accounted for 81.1% of all benign tumors. Adenoid cystic carcinoma comprised 32.9% of the malignant sample and was the most frequent malignant tumor. The principal location was the palate. Female was a little more of the benign and a male prevalence was observed on the malignant tumor. The mean age of patients with benign and malignant tumors were 40.9 and 49.1 years old, respectively (P < 0.05).</p><p><b>CONCLUSION</b>We consider extraordinary manifestation as histopathology, tumor, primary location, age and sex, presenting the results of a review of our experience with those minor salivary gland tumors.</p>

Infection of human papillomavirus in oral benign epithelial proliferation in children / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the presence of HPV infection of oral mucosa proliferative lesions in children and determine the associations of HPV types with oral mucosa lesions in children.</p><p><b>METHODS</b>Immunohistochemical method and in situ hybridization techniques were applied to detect human papillomavirus (HPV) infection in biopsies taken from clinical lesions in oral mucosa of 30 children.</p><p><b>RESULTS</b>The most frequent lesions detected were SCP (66.7%), followed by CA and FEH. The HPV viral antigen was present in 73.3% (22/30) of the oral benign epithelial proliferative lesions in children. A high frequency HPV was found in CA (6/6) and SCP (15/20) by means of IHC. In the ISH positive case, high risk HPV 16/18 was observed in 77.3% (17/22).</p><p><b>CONCLUSION</b>This study demonstrates a high prevalence of HPV infection in children's oral mucosa proliferative lesions, and high-risk HPV16/18 are predominant in children's oral mucosa proliferative lesions.</p>